PCR Quantification Detailed Report
PCR Base Line Subtracted Curve Fit Data (FAM)
Contains All Available Data 

General Data

Data File Name	SSI bacteria  2010-07-21 1524.opd
Data File Path	C:\Program Files\Bio-Rad\iQ5\Users\admin
Collected Data	Collected Data
Current Date	7/22/2010 10:19:14 AM
Run Date	7/21/2010 3:25:18 PM
User aborted the run	No
Active RMEs	Original
Active Well Factors	Dynamic 
Background Readings Valid	Yes
RME Valid	Yes
Well Factors Valid	Yes
Plate Setup File Name	SSI2010.pts
Plate Setup File Path	C:\Program Files\Bio-Rad\iQ5\Users\Guy
Protocol File Name	SSI2010.tmo
Protocol File Path	C:\Program Files\Bio-Rad\iQ5\SampleFiles
  	  
Computer name	NREL-QPCR
Created by app	iQ5.exe (v2.0.148.60623. (OS-Microsoft Windows NT 5.1.2600.0.Service Pack 2, CLR-1.1.4322.2463, Culture-en-US).)
Created by user	BioRad\admin
Creation Date	7/21/2010 3:25:18 PM
Created in Security Edition	No
Last Creation GUID	789d065f-9a2d-4424-8b1b-05695f424a36
Modified by user	BioRad\admin
Last modified date	7/21/2010 3:25:18 PM
OS Build and Service Pack	2600 (Service Pack 2)
Report differs from last save	Yes

Notes:   

Protocol:
	Cycle 1: (1X)
	Step 1:			95.0 °C			for 15:00.
	Cycle 2: (35X)
	Step 1:			94.0 °C			for 00:20.
	Step 2:			53.0 °C			for 00:30.
	Step 3:			72.0 °C			for 00:30.
	Data collection and real-time analysis enabled.

Modified Protocol:
	Protocol unchanged

PCR Quantification Data

PCR Amp/Cycle Chart

Standard Curve Data
	
Standard Curve Chart

	Fluor	PCR	R	Slope	y-Intercept
		Efficiency(%)	Squared		
	   FAM	  78.0	 0.993	-3.993	16.491

	Fluor	Units 	Quantity 	Original
		Changed?	Units	Units
	 FAM	  No	picograms	picograms

Number of valid standard wells:    None

Standard Curve Spreadsheet Data

	Fluor	Well	Type	Ident.	Rep	Ct	Log	SQ	SQ	SQ	Ct	Ct	Set
								SQ		Mean	SD	Mean	SD	Point
	FAM	A01	Std	-	1	N/A	3.954	9.00E+03	0.00E+00	0.00E+00	.00	0.000	N/A
	FAM	A02	Std	-	2	3.61	2.954	9.00E+02	9.00E+02	0.00E+00	4.93	1.138	N/A
	FAM	A03	Std	-	3	6.48	1.954	9.00E+01	9.00E+01	0.00E+00	8.16	1.456	N/A
	FAM	A04	Std	-	4	13.13	0.954	9.00E+00	9.00E+00	0.00E+00	12.82	0.272	N/A
	FAM	A05	Std	-	5	17.06	-0.046	9.00E-01	9.00E-01	0.00E+00	16.79	0.248	N/A
	FAM	A06	Std	-	6	21.08	-1.046	9.00E-02	9.00E-02	1.70E-17	20.84	0.248	N/A
	FAM	A07	Std	-	7	24.96	-2.046	9.00E-03	9.00E-03	0.00E+00	24.68	0.288	N/A
	FAM	A08	Std	-	8	28.91	-3.046	9.00E-04	9.00E-04	1.33E-19	28.50	0.358	N/A
	FAM	A09	NTC	-	1	31.34	N/A	0.00E+00	0.00E+00	0.00E+00	30.77	0.500	N/A
	FAM	B01	Std	-	1	N/A	3.954	9.00E+03	0.00E+00	0.00E+00	.00	0.000	N/A
	FAM	B02	Std	-	2	5.51	2.954	9.00E+02	9.00E+02	0.00E+00	4.93	1.138	N/A
	FAM	B03	Std	-	3	8.97	1.954	9.00E+01	9.00E+01	0.00E+00	8.16	1.456	N/A
	FAM	B04	Std	-	4	12.69	0.954	9.00E+00	9.00E+00	0.00E+00	12.82	0.272	N/A
	FAM	B05	Std	-	5	16.74	-0.046	9.00E-01	9.00E-01	0.00E+00	16.79	0.248	N/A
	FAM	B06	Std	-	6	20.85	-1.046	9.00E-02	9.00E-02	1.70E-17	20.84	0.248	N/A
	FAM	B07	Std	-	7	24.69	-2.046	9.00E-03	9.00E-03	0.00E+00	24.68	0.288	N/A
	FAM	B08	Std	-	8	28.36	-3.046	9.00E-04	9.00E-04	1.33E-19	28.50	0.358	N/A
	FAM	B09	NTC	-	1	30.41	N/A	0.00E+00	0.00E+00	0.00E+00	30.77	0.500	N/A
	FAM	C01	Std	-	1	N/A	3.954	9.00E+03	0.00E+00	0.00E+00	.00	0.000	N/A
	FAM	C02	Std	-	2	5.66	2.954	9.00E+02	9.00E+02	0.00E+00	4.93	1.138	N/A
	FAM	C03	Std	-	3	9.03	1.954	9.00E+01	9.00E+01	0.00E+00	8.16	1.456	N/A
	FAM	C04	Std	-	4	12.64	0.954	9.00E+00	9.00E+00	0.00E+00	12.82	0.272	N/A
	FAM	C05	Std	-	5	16.58	-0.046	9.00E-01	9.00E-01	0.00E+00	16.79	0.248	N/A
	FAM	C06	Std	-	6	20.58	-1.046	9.00E-02	9.00E-02	1.70E-17	20.84	0.248	N/A
	FAM	C07	Std	-	7	24.39	-2.046	9.00E-03	9.00E-03	0.00E+00	24.68	0.288	N/A
	FAM	C08	Std	-	8	28.24	-3.046	9.00E-04	9.00E-04	1.33E-19	28.50	0.358	N/A
	FAM	C09	NTC	-	1	30.57	N/A	0.00E+00	0.00E+00	0.00E+00	30.77	0.500	N/A
	FAM	D01	Unkn	-	3	17.86	-0.342	4.55E-01	8.24E-01	3.28E-01	16.94	0.804	N/A
	FAM	D02	Unkn	-	3	16.61	-0.031	9.32E-01	8.24E-01	3.28E-01	16.94	0.804	N/A
	FAM	D03	Unkn	-	3	16.35	0.035	1.08E+00	8.24E-01	3.28E-01	16.94	0.804	N/A
	FAM	D04	Unkn	-	5	15.55	0.236	1.72E+00	1.53E+00	1.74E-01	15.76	0.195	N/A
	FAM	D05	Unkn	-	5	15.94	0.139	1.38E+00	1.53E+00	1.74E-01	15.76	0.195	N/A
	FAM	D06	Unkn	-	5	15.79	0.177	1.50E+00	1.53E+00	1.74E-01	15.76	0.195	N/A
	FAM	D07	Unkn	-	7	17.34	-0.213	6.12E-01	6.01E-01	6.49E-02	17.38	0.191	N/A
	FAM	D08	Unkn	-	7	17.59	-0.275	5.31E-01	6.01E-01	6.49E-02	17.38	0.191	N/A
	FAM	D09	Unkn	-	7	17.21	-0.181	6.59E-01	6.01E-01	6.49E-02	17.38	0.191	N/A
	FAM	D10	Unkn	-	9	16.69	-0.051	8.90E-01	7.22E-01	2.86E-01	17.17	0.817	N/A
	FAM	D11	Unkn	-	9	16.70	-0.053	8.85E-01	7.22E-01	2.86E-01	17.17	0.817	N/A
	FAM	D12	Unkn	-	9	18.11	-0.406	3.92E-01	7.22E-01	2.86E-01	17.17	0.817	N/A
	FAM	E01	Unkn	-	2	19.71	-0.806	1.56E-01	3.44E-01	1.66E-01	18.52	1.037	N/A
	FAM	E02	Unkn	-	2	18.05	-0.389	4.08E-01	3.44E-01	1.66E-01	18.52	1.037	N/A
	FAM	E03	Unkn	-	2	17.81	-0.329	4.68E-01	3.44E-01	1.66E-01	18.52	1.037	N/A
	FAM	E04	Unkn	-	4	19.61	-0.781	1.66E-01	1.79E-01	2.25E-02	19.48	0.211	N/A
	FAM	E05	Unkn	-	4	19.24	-0.687	2.05E-01	1.79E-01	2.25E-02	19.48	0.211	N/A
	FAM	E06	Unkn	-	4	19.59	-0.777	1.67E-01	1.79E-01	2.25E-02	19.48	0.211	N/A
	FAM	E07	Unkn	-	8	17.27	-0.195	6.38E-01	6.55E-01	1.46E-02	17.23	0.039	N/A
	FAM	E08	Unkn	-	8	17.20	-0.178	6.63E-01	6.55E-01	1.46E-02	17.23	0.039	N/A
	FAM	E09	Unkn	-	8	17.20	-0.179	6.63E-01	6.55E-01	1.46E-02	17.23	0.039	N/A
	FAM	E10	Unkn	-	10	17.35	-0.216	6.08E-01	4.96E-01	2.01E-01	17.83	0.843	N/A
	FAM	E11	Unkn	-	10	17.33	-0.211	6.15E-01	4.96E-01	2.01E-01	17.83	0.843	N/A
	FAM	E12	Unkn	-	10	18.80	-0.579	2.64E-01	4.96E-01	2.01E-01	17.83	0.843	N/A
	FAM	F01	Unkn	-	6	26.95	-2.619	2.41E-03	3.48E-03	1.07E-03	26.36	0.556	N/A
	FAM	F02	Unkn	-	6	26.30	-2.457	3.49E-03	3.48E-03	1.07E-03	26.36	0.556	N/A
	FAM	F03	Unkn	-	6	25.84	-2.342	4.55E-03	3.48E-03	1.07E-03	26.36	0.556	N/A
	FAM	F04	Unkn	-	11	12.39	1.027	1.06E+01	1.27E+01	2.86E+00	12.11	0.395	N/A
	FAM	F05	Unkn	-	11	11.83	1.167	1.47E+01	1.27E+01	2.86E+00	12.11	0.395	N/A
	FAM	F06	Unkn	-	11	N/A	N/A	0.00E+00	0.00E+00	0.00E+00	.00	0.000	N/A
	FAM	F07	Unkn	-	12	13.09	0.852	7.11E+00	9.39E+00	1.97E+00	12.64	0.392	N/A
	FAM	F08	Unkn	-	12	12.41	1.022	1.05E+01	9.39E+00	1.97E+00	12.64	0.392	N/A
	FAM	F09	Unkn	-	12	12.41	1.023	1.05E+01	9.39E+00	1.97E+00	12.64	0.392	N/A
	FAM	F10	Unkn	-	14	13.06	0.858	7.22E+00	4.29E+00	2.80E+00	14.26	1.293	N/A
	FAM	F11	Unkn	-	14	14.08	0.603	4.01E+00	4.29E+00	2.80E+00	14.26	1.293	N/A
	FAM	F12	Unkn	-	14	15.63	0.215	1.64E+00	4.29E+00	2.80E+00	14.26	1.293	N/A
	FAM	G01	Unkn	-	1	16.03	0.115	1.30E+00	3.26E+00	1.71E+00	14.67	1.181	N/A
	FAM	G02	Unkn	-	1	14.09	0.602	4.00E+00	3.26E+00	1.71E+00	14.67	1.181	N/A
	FAM	G03	Unkn	-	1	13.90	0.650	4.47E+00	3.26E+00	1.71E+00	14.67	1.181	N/A
	FAM	G04	Unkn	-	15	12.70	0.949	8.89E+00	8.26E+00	1.54E+00	12.85	0.344	N/A
	FAM	G05	Unkn	-	15	13.25	0.813	6.50E+00	8.26E+00	1.54E+00	12.85	0.344	N/A
	FAM	G06	Unkn	-	15	12.61	0.972	9.37E+00	8.26E+00	1.54E+00	12.85	0.344	N/A
	FAM	G07	Unkn	-	16	16.48	0.002	1.01E+00	8.36E-01	1.60E-01	16.82	0.330	N/A
	FAM	G08	Unkn	-	16	17.14	-0.163	6.87E-01	8.36E-01	1.60E-01	16.82	0.330	N/A
	FAM	G09	Unkn	-	16	16.85	-0.089	8.14E-01	8.36E-01	1.60E-01	16.82	0.330	N/A
	FAM	G10	Unkn	-	13	14.26	0.558	3.62E+00	3.64E+00	1.11E+00	14.30	0.543	N/A
	FAM	G11	Unkn	-	13	14.87	0.407	2.55E+00	3.64E+00	1.11E+00	14.30	0.543	N/A
	FAM	G12	Unkn	-	13	13.78	0.678	4.76E+00	3.64E+00	1.11E+00	14.30	0.543	N/A

Run Parameters

	Hot Start?		No		
	Temperature Control Mode:    	Algorithmic     
	Volume:		25 ul

Data Analysis Parameters

Display Controls
	Fluor	Display Mode
	FAM	SinglePoint

Data Selection
	Fluor	Data Window 	Center
		Size
	FAM	99%	End

Digital Filtering
	Fluor	Global Filter	PCR Digital		Smoothing Filter 
		Enabled?	Filter Type		Desired Width
	FAM	Off	Weighted Mean		  5
	
PCR Data Analysis Method	
	Fluor	Data Analysis Method
	FAM	PCR Base Line Subtracted Curve Fit

PCR Baseline Data Analysis Parameters

Baseline Calculation
	Fluor	Baseline	Auto Baseline		Global Baseline Cycles
		 Method	Cycle Calculation?	Start	End
	  FAM	Data Window	  Yes		  N/A	  N/A

Overriden Baseline Cycles	None

Threshold Calculation
	Fluor	Use Auto	Auto Calculated	User Defined 
		Threshold?	Threshold Value	Threshold Value
	 FAM	 Yes	327.38		670.31

Excluded Wells
	Excluded Well Count:    	0

		
 
Modified Wells
	Modified Well Count:	0

 			 	 	 
End